We narrowed to 4,504 results for: pcr

1. pFA6a-GFP(S65T)-kanMX6

   Plasmid

   #39292

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-kanMX6-PGAL1

   Plasmid

   #41605

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-His3MX6-PGAL1

   Plasmid

   #41607

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKF257

   Plasmid

   #52533

   Purpose

   expresses I-SceI nuclease in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Tags

   HA and NLS

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. STAGR_gRNAScaffold_h7SK

   Plasmid

   #102842

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_h7SKpromoter
   

   Use

   As pcr template

   Tags

   Expression

   Mutation

   Promoter

   h7SK

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR-SUMO1

   Plasmid

   #114388

   Purpose

   pDONR vector with SUMO1 gene

   Depositor

   Vincent Dion

   Article

   Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q29H compared with NCBI reference NP_003343.1

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHyg-His6

   Plasmid

   #212776

   Purpose

   C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMCRW2

   Plasmid

   #127380

   Purpose

   pBC with 4 repeats of 80bp DNA template

   Depositor

   Richard C. Willson

   Article

   Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

   Insert

   4 repeats of 80 bp DNA template sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pG-HIV-SIN-2LTR

   Plasmid

   #104591

   Purpose

   Standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   2LTR circle junction of self-inactivating HIV-based vectors
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-TRP32

    Plasmid

    #71730

    Purpose

    inducible expression of TRP32 in E.coli

    Depositor

    Jere McBride

    Article

    Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

    Insert

    TRP32
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pFA6a-GFP(S65T)-His3MX6

    Plasmid

    #41598

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHyg-yeGFP-His6

    Plasmid

    #212781

    Purpose

    C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-kanMX6-P3nmt1

    Plasmid

    #39280

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. STAgR_Neo

    Plasmid

    #102992

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-His3MX6-PGAL1-GFP

    Plasmid

    #41616

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-kanMX6-PGAL1-3HA

    Plasmid

    #41608

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRB14

    Plasmid

    #52522

    Purpose

    expresses a myc-tagged version of hCas9 in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    S. pyogenes cas9 with humanized codon bias
    

    Use

    Tags

    myc

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge