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pAAV_BiCHATe27_C1V1_eYFP

Plasmid

#213831

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_jGCaMP8m

Plasmid

#213918

Purpose

AAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8m

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV1

Insert

jGCaMP8m

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_Gq_dTomato

Plasmid

#213938

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p112A1NE

Plasmid

#85983

Purpose

For construction of cDNA expression library.

Depositor

Wolf Frommer

Article

Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

Type

Empty backbone

Expression

Yeast

Promoter

Adh1

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pXP722

Plasmid

#46056

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXR003_puroR

Plasmid

#219819

Purpose

hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

Depositor

Philipp Maass

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z18-ehaA_LacI

Plasmid

#186314

Purpose

Plasmid for inducible E coli expression of the Z18 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z18-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pC210

Plasmid

#65950

Purpose

Weak Repressor plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

CinI

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-w

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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TLD_062

Plasmid

#19698

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_GCaMP6f

Plasmid

#213859

Purpose

AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_dTomato_nlsdTomato

Plasmid

#218792

Purpose

AAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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KW57_HART_RAC1

Plasmid

#207399

Purpose

Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7

Plasmid

#175747

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6 and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 25043-35938

Use

Adenoviral and Synthetic Biology

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Tags

MGH

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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