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pMCSF-R(mA)-luc
(Plasmid #12421)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12421 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M-CSF receptor promoter fragment
  • Alt name
    M-CSF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Mutation
    C/EBP binding site mutation (created a KpnI site in the mutation site)
  • Entrez Gene
    CSF1R (a.k.a. C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSF-R(mA)-luc was a gift from Dong-Er Zhang (Addgene plasmid # 12421 ; http://n2t.net/addgene:12421 ; RRID:Addgene_12421)
  • For your References section:

    CCAAT enhancer-binding protein (C/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter. Zhang DE, Hetherington CJ, Meyers S, Rhoades KL, Larson CJ, Chen HM, Hiebert SW, Tenen DG. Mol Cell Biol. 1996 Mar . 16(3):1231-40. PubMed 8622667