We narrowed to 10,453 results for: yeast

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159K

Plasmid

#123626

Purpose

Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Lys

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 H12A

Plasmid

#123619

Purpose

Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

His12Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47A

Plasmid

#123620

Purpose

Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47K

Plasmid

#123621

Purpose

Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Lys

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127A

Plasmid

#123622

Purpose

Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127Q

Plasmid

#123623

Purpose

Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Gln

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159A

Plasmid

#123625

Purpose

Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Ala

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (L313D I314K)

Plasmid

#121409

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to …

Promoter

CUP1 promoter

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

Plasmid

#117663

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT2)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE724

Plasmid

#103023

Purpose

expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crPDR12-3.S (PDR12 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HCKan-T-TEF2

Plasmid

#65340

Purpose

construction of TEF2 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 terminator

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TDH3

Plasmid

#65336

Purpose

construction of TDH3 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3 promoter

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-ADH1

Plasmid

#65339

Purpose

construction of ADH1 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ADH1 terminator

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-CYC1

Plasmid

#65338

Purpose

construction of CYC1 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1 promoter

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPEasy9R

Plasmid

#218291

Purpose

Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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