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YEE_RFP

Plasmid

#228185

Purpose

Yeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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p39.pA.NGFR.mCMV.hPGK.SrtA(Pasqual).WPRE[sequenced]

Plasmid

#226758

Purpose

secreted SrtA in p36

Depositor

Ferdinando Pucci

Publication

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Expression

Mammalian

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-Cut190*SS

Plasmid

#218706

Purpose

Encodes codon optimized Cut190*SSenzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

Cut190*SS

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-SfCut

Plasmid

#218708

Purpose

Encodes codon optimized SfCut enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

SfCut

Tags

10xHis

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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p35.pA.NGFR.mCMV.hPGK.mSrtA(Pasqual)TM.WPRE[sequenced]

Plasmid

#226760

Purpose

sortase A (Pasqual)- TM in p36

Depositor

Ferdinando Pucci

Publication

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Expression

Mammalian

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-tkUnaG

Plasmid

#221661

Purpose

For Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2

Expression

Plant

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM294

Plasmid

#226130

Purpose

Expression of Ufd1-3C-His6

Depositor

Andreas Martin

Publication

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1-3C-His6

Expression

Bacterial

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-mRFP

Plasmid

#218709

Purpose

Encodes codon optimized mRFP enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

mRFP

Tags

10xHis

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-A2

Plasmid

#218691

Purpose

Encodes codon optimized LCC-A2 enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-A2

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-PES-H1

Plasmid

#218704

Purpose

Encodes codon optimized PES-H1 enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

PES-H1

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_1_WPRE

Plasmid

#209765

Purpose

AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Use

AAV

Expression

Mammalian

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Expression

Mammalian

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-miniSOX

Plasmid

#206366

Purpose

Expresses miniSOX in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Publication

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

miniSOX

Use

Lentiviral

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM_mePOU_3xFlag

Plasmid

#206395

Purpose

Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Publication

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Expression

Bacterial

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2014-Cas12i2

Plasmid

#188275

Purpose

prokaryotic expression

Depositor

Wei Li

Publication

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_iRFP713

Plasmid

#167884

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_Puro

Plasmid

#167879

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_iRFP713

Plasmid

#167876

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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