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pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AAA)

Plasmid

#24241

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
NHR1-Flag pcDNA3.1

Plasmid

#17315

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pUAS-Tum

Plasmid

#37304

Depositor

Norbert Perrimon

Article

Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

Insert

hopTum-1 (hop Fly)

Expression

Insect

Mutation

Tul-l mutation is a G to A transversion at nucleo…

Promoter

Hsp70

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
Neurl1-FLAG/Jag1-HA pIRES

Plasmid

#17337

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1G2A-Flag pcDNA3.1

Plasmid

#17320

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Gly 2 changed to Ala

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pN1_FV-NUP62-EGFP3

Plasmid

#252747

Purpose

Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore in absence of Rapalog2.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

3xEGFP and FV

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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Modified pYTK075

Plasmid

#254020

Purpose

A part plasmid designed to use the yeast selective marker (LEU2 gene) as a Level 1 TU fragment.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LEU2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-ATG13-HF|DD

Plasmid

#249535

Purpose

Expressing human full length ATG13 with H214D/F215D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H214D and F215D

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG101ΔCTH (WF|DD)

Plasmid

#250341

Purpose

Expressing human ATG101ΔCTH with W110D and F112D mutation

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG101 (ATG101 Human)

Tags

strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG101(WF|DD)

Plasmid

#250342

Purpose

Expressing human full length ATG101 with W110D and F112D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG101 (ATG101 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230

Plasmid

#250343

Purpose

Expressing human ATG13 (aa. 1-230)

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_Rev-GR(m10)-EGFP

Plasmid

#252751

Purpose

Control for studying nuclear export. Expression of a fusion protein containing full-length Rev, the hormone-responsive element of the rat Gr, and GFP. The m10 mutation abolishes nuclear export.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

HIV-1 Rev and Glucocorticoid Receptor (GR)

Tags

EGFP

Expression

Mammalian

Mutation

m10 is mutation of nuclear export sequence

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pYO1704

Plasmid

#252704

Purpose

Protein expression of GST-HsLC3B (1-120) in bacteria

Depositor

Roger Williams

Article

van Vliet et al J Cell Biol. 2026 Mar 2;225(3):e20250708

Insert

GST-HsLC3B (1-120) (MAP1LC3B Human)

Expression

Bacterial

Mutation

1-120

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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IR InterIR gRNA #5

Plasmid

#247547

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region between inverted repeats of Distal Junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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