We narrowed to 24,795 results for: crispr

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pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

Plasmid

#196847

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

Plasmid

#196844

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJAT56

Plasmid

#204300

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EGFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh5-2300

Plasmid

#171788

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO 9.6_bCas9-CP1010

Plasmid

#121498

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.15_bCas9-CP1249

Plasmid

#121500

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 9.16_bCas9-CP1282

Plasmid

#121501

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO43.3_human ProCas9Flavi

Plasmid

#121629

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJF121

Plasmid

#113325

Purpose

pTet-W108 scRNA.b2

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCD005

Plasmid

#113314

Purpose

W108 scRNA

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2

Use

CRISPR

Expression

Bacterial

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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hNTo1-qgRNA-pYJA5

Plasmid

#217781

Purpose

Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Insert

Quadruple sgRNA-expression cassette for nontargeting control for gene ablation

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, mouse U6, human H1, human 7SK

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-ABE8e-dCas9-BSD

Plasmid

#226116

Purpose

Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10622

Plasmid

#239274

Purpose

Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE5max-SF3B1-K700E

Plasmid

#200317

Purpose

Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Inserts

K700E-ng1+pg2
PEmax-2A-MLH1dn

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

Plasmid

#196971

Purpose

Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Inserts

Cas9-RT
rtTA3G-P2A-eGFP

Expression

Mammalian

Promoter

PGK and TREG3G

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z11

Plasmid

#120054

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKMDDV_Plasmid_1

Plasmid

#249037

Purpose

Plasmid 1 containing constitutively expressed dCas9 and RBP-AD along with inducible scRNA_RFP

Depositor

Domitilla Del Vecchio

Article

Aravind et al Cell Systems, 2026; 17

Inserts

dCas9
MCP-SoxS(R93A/S101A)
scRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23107, BBa_J23116, and PphlF

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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