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VV246: CoChR(C108S)-GCaMP6f-ER in fck

Plasmid

#58521

Purpose

Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn3

Plasmid

#59359

Purpose

Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

Plasmid

#80860

Purpose

Phagemid (encodes evolved cI5C6A,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL-neo Myr-FLAG-p110alpha

Plasmid

#10890

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

p110alpha (PIK3CA Bovine)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCfB4848

Plasmid

#106138

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM24951

Plasmid

#169088

Purpose

MoClo level 0 Tomato E8 promoter module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Tomato E8 promoter

Use

Synthetic Biology; Moclo cloning vector

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pB1H2w5-12-C81S

Plasmid

#18043

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert between Kpn1 and Xba1

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEGFPC1-Sclt1

Plasmid

#128873

Purpose

For mammalian expression of mouse Sclt1

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Sclt1 (Sclt1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMM0553

Plasmid

#128989

Purpose

LEU2 integration vector of pZF(3BS)-mRUBY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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