We narrowed to 19,081 results for: cat

Showing: 10301 - 10320 of 19081 results

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pB-puro-mU6-CNPsgRNA

Plasmid

#126610

Purpose

Expresses CNP sgRNA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP sgRNA (Cnp Mouse)

Use

CRISPR

Promoter

mouse U6 promoter

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRAN357

Plasmid

#120898

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDDIT3.1.0-gDNA

Plasmid

#112396

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3

Depositor

Kevin White

Insert

DDIT3 (DDIT3 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pURD214

Plasmid

#113871

Purpose

expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA5 HXT13-15-16 sgRNA2-HXT2

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-D4J3S7

Plasmid

#103096

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-hTIG3-1-164

Plasmid

#100724

Purpose

Full-length human TIG3 in E.coli expression vector

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

RARRES3 (PLAAT4 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-hTIG3-1-132

Plasmid

#100720

Purpose

Expression of N-terminal region of human TIG3 in E.coli

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

RARRES3 (PLAAT4 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4a (mEosFP4a-V69T)

Plasmid

#99225

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4a

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K164R)

Plasmid

#99544

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC E254D

Plasmid

#100033

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC E254D

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E254D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only
tet-pLKO shMKP7.v1 puro

Plasmid

#87794

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo-PAX9

Plasmid

#75091

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestRFP_HuC

Plasmid

#65765

Purpose

expresses RFP-tagged HuC in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL3 (ELAVL3 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only