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miR-206-1/miR-133b promoter GFP

Plasmid

#20793

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-206-1/mir-133b (mir206-1 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pICH42222

Plasmid

#50328

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation; See De…

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C0-GFP

Plasmid

#46040

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2006 Aug 25;281(34):24662-7

Insert

nonmuscle myosin heavy chain II-C, isoform C0 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pGJJ055

Plasmid

#183622

Purpose

ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Profilin-10

Plasmid

#55440

Purpose

Localization: Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Profilin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSuperRetro human Hic-5

Plasmid

#11188

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (TGFB1I1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pQStrep2-PIR

Plasmid

#31570

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

pirin (PIR Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Article

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-SOX2

Plasmid

#115687

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MD-SOX2

Use

Lentiviral

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pLO101

Plasmid

#90221

Purpose

Complements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_07102

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLO102

Plasmid

#90222

Purpose

Complements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)

Depositor

Berl Oakley

Article

Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

Insert

ATET_02347

Use

Aspergillus

Promoter

pLac

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1166

Plasmid

#129520

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Hox A13a

Plasmid

#36463

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13a (hoxa13a Zebrafish)

Use

Riboprobe synthesis

Mutation

contains aa102-290; M107T

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pT2iU6m5Hoxa13bE4PCh(#765)

Plasmid

#184102

Purpose

cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis

Depositor

Sophie Vriz

Insert

5myc-ZfHoxa13b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes) + in vitro tra…

Tags

5xMyc

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi+SP6

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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