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pILGFP82

Plasmid

#83541

Purpose

Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

YEF3 promoter-yEGFP

Expression

Yeast

Promoter

YEF3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP80

Plasmid

#83540

Purpose

Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL3 promoter-yEGFP

Expression

Yeast

Promoter

RPL3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP78

Plasmid

#83539

Purpose

Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSB1 promoter-yEGFP

Expression

Yeast

Promoter

SSB1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-lin26

Plasmid

#80357

Purpose

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR

Expression

Worm

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-atg4.2

Plasmid

#80352

Purpose

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR

Expression

Worm

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-set6

Plasmid

#80362

Purpose

Pmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::set-6 3'UTR

Expression

Worm

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-ets4

Plasmid

#80355

Purpose

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR

Expression

Worm

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mex3

Plasmid

#80361

Purpose

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR

Expression

Worm

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mbk2

Plasmid

#80360

Purpose

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR

Expression

Worm

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

Plasmid

#80325

Purpose

Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Publication

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Tags

Biotin

Expression

Mammalian

Mutation

H170A and H256A

Promoter

CMV

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

Plasmid

#72924

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Serine#37 changed to Alanine;Silent mutations mad…

Promoter

TRE tight

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-S37A-GATA6-3XAU1

Plasmid

#72613

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Mutation

Changed serine37 to alanine

Promoter

TRE tight promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

Plasmid

#72617

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

Serine#37 changed to Alanine;Silent mutations mad…

Promoter

TRE tight

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)

Plasmid

#70199

Purpose

Promoter panel

Depositor

Richard Murray

Publication

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFusX4-41

Plasmid

#63549

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NN NI

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusX4-62

Plasmid

#63550

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NG NG HD

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusX4-63

Plasmid

#63551

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NG NG NN

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusX4-64

Plasmid

#63552

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NG NG NG

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusB3-36

Plasmid

#63553

Purpose

Encodes RVD positions 13-15 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NI NG

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusB3-47

Plasmid

#63554

Purpose

Encodes RVD positions 13-15 in TALEN assembly

Depositor

Stephen Ekker

Publication

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NG NN

Use

TALEN

Mutation

none

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only