We narrowed to 15,231 results for: LEA

1. pDZ585 pKAN 2x-mCherry

   Plasmid

   #72236

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   2x-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-GFP-AsLOV2-NES27 (pDN158)

   Plasmid

   #72659

   Purpose

   Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   H2B-GFP-AsLOV2-NES27
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREP3-TEV-NLS

   Plasmid

   #21146

   Depositor

   Stephen Kearsey

   Article

   Yang et al BMC Mol Biol. 2005 . 6():13.

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   S. pombe

   Tags

   2xNLS, NLS, and myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Banshee-Sh-Ctrl

   Plasmid

   #85587

   Purpose

   Control hairpin sequence used in gene knockdown experiements

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   sh-Ctrl
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

   Plasmid

   #72230

   Purpose

   Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   PP7-PS-2x-yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

   Plasmid

   #72234

   Purpose

   Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   PP7-PS-2x-yeGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADE3p

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPKAnes

   Plasmid

   #61092

   Purpose

   GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

   Depositor

   Steven Green

   Article

   Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

   Insert

   pkac2
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-CMV-SMCHD1-R1868*-3xFLAG

   Plasmid

   #130679

   Purpose

   Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 R1868* (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R1868_V2005delinsX

   Promoter

   CMV

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

   Plasmid

   #130688

   Purpose

   Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon37-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   1M_T1522delinsM

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

    Plasmid

    #130681

    Purpose

    Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-36 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1523_V2005delinsX

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDESTFLAGHApuro3-Ago Chi-a

    Plasmid

    #14075

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-a
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-a

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72233

    Purpose

    Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDESTFLAGHApuro3-Ago Chi-b

    Plasmid

    #14085

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-b
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-b

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pDESTFLAGHApuro3-Ago Chi-c

    Plasmid

    #14086

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-c
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-c

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pDESTFLAGHApuro3-Ago Chi-d

    Plasmid

    #14087

    Depositor

    Thomas Tuschl

    Article

    Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

    Insert

    Ago Chi-d
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Ago Chimera-d

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a(+)-T7-OmpA-MNaseB-6xHis

    Plasmid

    #214809

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease B
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS2699-CMV-Nme2Cas9-E932R

    Plasmid

    #228720

    Purpose

    Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-E932R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid

    #228717

    Purpose

    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-D56R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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