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pLenti-HF1RA-P2A-Puro

Plasmid

#110853

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mGSDMD1-251

Plasmid

#111565

Purpose

Express ELANE-cleaved mouse GSDMD in mammalian cells

Depositor

Hongbo Luo

Publication

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only
pSCBe

Plasmid

#110065

Purpose

Trc1O promoter express gene in Synechocystis 6803

Depositor

Devaki Bhaya

Type

Empty backbone

Tags

3' end His tag and 5' end FLAG tag

Expression

Bacterial

Promoter

Trc1O

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL-L1-Tf1-Ctrl

Plasmid

#103036

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-Tf1

Use

T3 in vitro transcription for expression in mamma…

Tags

FLAG and HA

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Publication

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative (Y699F)

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate

Plasmid

#79596

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pVITRO1-SS-345

Plasmid

#68785

Purpose

R->A Sensor pair 1 with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

C-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry

Tags

NLS, flag, and nls

Expression

Mammalian

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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TAAR2-Tango

Plasmid

#66509

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TAAR2 (TAAR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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P2RY11-Tango

Plasmid

#66470

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P2RY11 (P2RY11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
MRGPRG-Tango

Plasmid

#66438

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MRGPRG (MRGPRG Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
NMUR1-Tango

Plasmid

#66446

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NMUR1 (NMUR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
NPS-Tango

Plasmid

#66452

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NPS (NPSR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
HTR5-Tango

Plasmid

#66413

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTR5

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR19-Tango

Plasmid

#66343

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR19 (GPR19 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR22-Tango

Plasmid

#66346

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR22 (GPR22 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR34-Tango

Plasmid

#66353

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR34 (GPR34 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR45-Tango

Plasmid

#66360

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR45 (GPR45 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR12-Tango

Plasmid

#66309

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR12 (GPR12 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only