We narrowed to 15,772 results for: LEA

1. pGBW-m4046686

   Plasmid

   #149150

   Purpose

   Yeast Expression plasmid for lSARS-CoV-2 eader protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 leader protein (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046560

   Plasmid

   #145471

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046493

   Plasmid

   #145533

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046229

   Plasmid

   #145569

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046489

   Plasmid

   #145554

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046335

   Plasmid

   #145534

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046346

   Plasmid

   #145390

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. SPL10p::pCGTAG (SPL10p::NLS-GFP)

   Plasmid

   #27410

   Depositor

   David Bartel

   Article

   Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

   Insert

   SPL10 promoter (AT1G27370 Mustard Weed)
   

   Mutation

   SPL10 promoter transcriptionally fused to a nucle…

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. wt Cus2p

   Plasmid

   #73046

   Purpose

   Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Promoter

   T7

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG607

    Plasmid

    #40193

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (mex-5 Nematode)
    

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

    Expression

    Worm

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pJA14

    Plasmid

    #59929

    Purpose

    gRNA for cleavage at rde-1(H974) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rde-1(H974) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST50Trc2-CBPDHFR

    Plasmid

    #63954

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Calmodulin Binding Peptide (CBP)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5I3L (DPF3)

    Plasmid

    #79522

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DPF3 (DPF3 Human)
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pST50Trc4-CBPDHFR

    Plasmid

    #63990

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Calmodulin Binding Peptide (CBP)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXNAC3H

    Plasmid

    #47074

    Purpose

    FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sus1/PET32a

    Plasmid

    #26963

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    Sus1 (SUS1 Budding Yeast)
    

    Tags

    His6 and Trx

    Expression

    Bacterial

    Mutation

    with TEV site to cleave the N-terminal his+Trx tag

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMTB-Multibow-hO

    Plasmid

    #60996

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKO

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    mKO
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Promoter

    Bactin2

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAGM5331

    Plasmid

    #50294

    Depositor

    Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC-GW-Kan-VPC

    Plasmid

    #244163

    Purpose

    Reporter for human p38alpha activation state by FRET

    Depositor

    Thomas Kuhlman

    Insert

    MAPK14/p38a (MAPK14 Human)
    

    Use

    Synthetic Biology

    Tags

    mCerulean3 and mVenus

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUW H2B-mCherry-P2A-PuroR

    Plasmid

    #239554

    Purpose

    Expression and nuclear localization of mCherry

    Depositor

    Tim Ahfeldt, Joel Blanchard

    Article

    et al iScience 28 (2025)

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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