We narrowed to 15,772 results for: LEA

1. pCHAP5

   Plasmid

   #206851

   Purpose

   A plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

   Depositor

   Bogumil Karas

   Article

   Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-6xHis-TrxF

   Plasmid

   #207454

   Purpose

   E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

   Depositor

   Joseph Jez, Sarah Stainbrook

   Article

   Stainbrook et al bioRxiv 2022.08.24.505184

   Insert

   Thioredoxin F
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   T7

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPTM p62 PB1 (1-122)

   Plasmid

   #210428

   Purpose

   Bacterial expression of MBP-PB1(1-122) with TEV cleavage site

   Depositor

   Carsten Sachse

   Article

   Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

   Insert

   p62 PB1 (1-122) (NUP62 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. C-SWAT 3MYC SMASh

   Plasmid

   #182524

   Purpose

   C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   SMASh Tag
   

   Tags

   3MYC

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-SWAT 3MYC SMASh

   Plasmid

   #182525

   Purpose

   C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   SMASh Tag
   

   Tags

   3MYC

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-LgBiT-GAI

   Plasmid

   #183405

   Purpose

   Expresses nuclear-localized LgBiT-GAI

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-GAI
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMVTO-gfp-Kir2.1-HA-tevs-KKYL

   Plasmid

   #179706

   Purpose

   TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Kir2.1
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRMCD111

   Plasmid

   #172957

   Purpose

   pRMCD77 encoding Wzz mutant G311A

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

   Insert

   Shigella flexneri wzz G311A mutant gene
   

   Expression

   Bacterial

   Mutation

   D168G and N33D- please see depositor comment

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRMCD113

   Plasmid

   #172959

   Purpose

   pRMCD77 encoding Wzz mutant G305A/G311A

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

   Insert

   Shigella flexneri wzz G305A/G311A mutant gene
   

   Expression

   Bacterial

   Mutation

   G305A/G311A, A208V - please see depositor comments

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSETA_crG18

    Plasmid

    #172930

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorG18
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD0CM0549

    Plasmid

    #162300

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, SUMO-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPQD0CM0550

    Plasmid

    #162301

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-SUMO-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPMY0SP0002

    Plasmid

    #162304

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, 6xHis tag-[HRV 3C cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPQD0CM0551

    Plasmid

    #162303

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPQD0CM0547

    Plasmid

    #162298

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPQD0CM0546

    Plasmid

    #162297

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0CM0544

    Plasmid

    #162295

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPQD0CM0543

    Plasmid

    #162294

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD0CM0541

    Plasmid

    #162292

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[thrombin cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-nls

    Plasmid

    #108882

    Purpose

    Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls
    

    Use

    Multisite gateway

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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