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pcDuex2-rsTagRFP

Plasmid

#205757

Purpose

Expression of rsTagRFP in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsTagRFP

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PMT-mCherry

Plasmid

#203781

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry

Expression

Mammalian

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mCherry_2

Plasmid

#203782

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry dimer

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSA102

Plasmid

#59788

Purpose

drives mCherry in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
mCherry (worm)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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HRE-dUnOHR

Plasmid

#201714

Purpose

Hypoxia responsive promoter driving UnaG and mOrange

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsEGFP2-E

Plasmid

#205753

Purpose

Expression of rsEGFP2-E in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsEGFP2-E

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise

Plasmid

#103972

Purpose

Plasmid encoding mTurquoise

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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LifeAct-SNAPf-mTurquoise2

Plasmid

#197497

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in actin filaments.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-2ndVal mGFPmut2

Plasmid

#104033

Purpose

Plasmid encoding 2ndVal mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

mGFPmut2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-A75-RZ-URA3

Plasmid

#130874

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

Depositor

Chang Liu

Article

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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HA-RNF126

Plasmid

#138647

Purpose

Mammalian expression of 2HA-tagged RNF126

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Tags

HA-HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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