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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MS2-epegRNA-Dnmt1

Plasmid

#211372

Purpose

Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MS2-epegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSB-BbsI-sgRNA

Plasmid

#203359

Purpose

Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

BbsI Cut Sites

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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DGKQ gRNA (BRDN0001162275)

Plasmid

#77377

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKQ

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKQ (DGKQ Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K3 gRNA (BRDN0001148097)

Plasmid

#76016

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K3 (MAP3K3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-eGFP

Plasmid

#116926

Purpose

Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

Depositor

Diane Mathis, Christophe Benoist

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_blast-tagBFP-lox5171

Plasmid

#162072

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_VCL sgRNA / hSpCas9

Plasmid

#172837

Purpose

Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_blast-tagBFP-lox2272

Plasmid

#162071

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox5171

Plasmid

#162070

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MAGI1 gRNA (BRDN0001146833)

Plasmid

#78067

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAGI1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAGI1 (MAGI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAGI1 gRNA (BRDN0001487062)

Plasmid

#78068

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAGI1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAGI1 (MAGI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAGI1 gRNA (BRDN0001146934)

Plasmid

#78069

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAGI1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAGI1 (MAGI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAGI1 gRNA (BRDN0001147011)

Plasmid

#78070

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAGI1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAGI1 (MAGI1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge