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We narrowed to 16,183 results for: nes

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Showing: 10741 - 10760 of 16183 results

  2. pJK422

    Plasmid
    #155373
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155373-plasmid-map-sequence-id-319760

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  3. pJK423

    Plasmid
    #155374
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155374-plasmid-map-sequence-id-319753

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  5. JEV-ED3_C5I

    Plasmid
    #251260
    Purpose
    To express the Japanese encephalitis virus envelope protein domain III with a five-isoleucine tag at the C-terminus in E. coli
    Depositor
    Yutaka Kuroda
    Publication
    Islam et al Vaccine. 2025 May 22;56:127143. doi: 10.
    Insert
    JEV-ED3_C5I
    Tags
    5xIle with a GG linker and 6xHis, 11 aa linker, t…
    Expression
    Bacterial
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    251260-plasmid-map-sequence-id-508114

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  6. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

    Plasmid
    #199253
    Purpose
    Expression construct encoding the high specificity SpCas9-KA-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199253-plasmid-map-sequence-id-391616

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  7. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Publication
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  8. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    155378-plasmid-map-sequence-id-324490

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  17. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  18. pLVX ALIX-GFP S718/721A

    Plasmid
    #138583
    Purpose
    Expresses fluorescent ALIX S718/721A in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to alanines
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138583-plasmid-map-sequence-id-322303

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  19. INT_GFPsp

    Plasmid
    #212144
    Purpose
    Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Expression
    Bacterial
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212144-plasmid-map-sequence-id-420757

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  20. INT_GFPsp_CamRcargo

    Plasmid
    #212143
    Purpose
    Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
    Expression
    Bacterial
    Promoter
    pJ23119
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212143-plasmid-map-sequence-id-420641

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Showing: 10741 - 10760 of 16183 results