We narrowed to 15,984 results for: grna

1. pJMP8923

   Plasmid

   #220904

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEmCherry2

   Plasmid

   #118413

   Purpose

   Red fluorescent reporter for assessing CRISPR gRNA activity

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-RFP-shCntrl

   Plasmid

   #69040

   Purpose

   Lentiviral expression of control shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   control shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUPER-scramble

   Plasmid

   #118349

   Purpose

   This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   scrambled shRNA
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Aurora A

   Plasmid

   #198402

   Purpose

   Expression of EGFP-tagged Aurora A in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Aurora A
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Expression

   Bacterial

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT09

   Plasmid

   #223381

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-entry-puro

   Plasmid

   #85745

   Purpose

   Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRa_VPR_yl

    Plasmid

    #107677

    Purpose

    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

    Inserts

    Codon optimized dCas9-VPR
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYS2.1-crtYf

    Plasmid

    #89077

    Purpose

    Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYF
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1 puro GFP siRNA

    Plasmid

    #12273

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    GFP siRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL-mU6-GFPGO2-IRES-mScarletI

    Plasmid

    #136896

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    2xNLS on GFP, 3xNLS on mScarlet

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQAT01

    Plasmid

    #223373

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAf-CRISPR-yA

    Plasmid

    #191015

    Purpose

    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Insert

    yA
    

    Use

    CRISPR

    Promoter

    Aspergillus flavus U6

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA)

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRIS

    Plasmid

    #120424

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3, IS150
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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