We narrowed to 15,772 results for: LEA

1. pBA2675

   Plasmid

   #189997

   Purpose

   Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

   Depositor

   Bungo Akiyoshi

   Article

   Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

   Inserts

   FBP75
   VhhGFP4
   

   Use

   Expression vector for trypanosoma brucei

   Tags

   NLS

   Mutation

   N-terminal 600 bp of FBP75

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-GFP-P2A

   Plasmid

   #80807

   Purpose

   GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFP-P2A
   

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. VKG1-gRNA-pX330

   Plasmid

   #108671

   Purpose

   Cleavage of VKG1 sequence by CRISPR/Cas9

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   gRNA for VKG1 sequence
   

   Use

   CRISPR

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCW02

   Plasmid

   #72301

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET26b_Nb.AT110.i1

   Plasmid

   #135481

   Purpose

   E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110.i1
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1938

   Plasmid

   #82563

   Purpose

   High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

   Insert

   Ple261
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Available Since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDEST14-DoubleCatcher-⍺-Lock

   Plasmid

   #216286

   Purpose

   Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ⍺-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-DoubleCatcher-β-Lock

   Plasmid

   #216287

   Purpose

   Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher β-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECFP-DEVG-Venus

    Plasmid

    #24538

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVG-Venus
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    DEVDR mutated to DEVG

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

14. JAK3 (JAK3A-c023)

    Plasmid

    #98229

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JAK3A (JAK3 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam00069:Pkinase 822-1091

    Promoter

    polyhedrin

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-puro-NLS-LSSmKate2

    Plasmid

    #34586

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate2
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF mito-cGFP (matrix)

    Plasmid

    #188904

    Purpose

    Expresses mitochondrial GFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

    Insert

    mitochondrial GFP
    

    Tags

    Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

    Expression

    Mammalian

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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