We narrowed to 15,772 results for: LEA

1. MECR

   Plasmid

   #38850

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MECR (Mecr Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4046203

   Plasmid

   #145488

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. csr-1_son-1_vsfGFP9

   Plasmid

   #191747

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and vsfGFP-9

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-FAK0

   Plasmid

   #59128

   Purpose

   Cloning vector for FAK fret sensor without helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUE1-TSP-UL32

   Plasmid

   #162658

   Purpose

   Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

   Plasmid

   #154399

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   SARS-CoV2 N Protein (N SARS-CoV2)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOG824

   Plasmid

   #105401

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   SV40 nuclear localization signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   March 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEG476

   Plasmid

   #40198

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (S404A) (mex-5 Nematode)
   

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   S404A

   Promoter

   pie-1

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pRATIO2214

    Plasmid

    #141303

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVITRO1-BFP-LaminB1

    Plasmid

    #184460

    Purpose

    Nuclear membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. hOtub1

    Plasmid

    #26959

    Depositor

    Cynthia Wolberger

    Article

    Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

    Insert

    Otub1 (OTUB1 Human)
    

    Tags

    His6 (TEV cleavable)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

13. CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

    Plasmid

    #179646

    Purpose

    TVMVP-inducible IL-12 RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    IL12 A/B
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. [GG][Pin]

    Plasmid

    #97191

    Purpose

    Pin nuclease domain with Pum cloning site

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    Pin domain
    

    Use

    Synthetic Biology

    Tags

    Pum cloning site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMEXC3GH (Hyg)

    Plasmid

    #110082

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRATIO4214

    Plasmid

    #141314

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PA-mCherry1-LaminA-C-18

    Plasmid

    #57185

    Purpose

    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LaminA (LMNA Human)
    

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only