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We narrowed to 12,029 results for: LEA

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Showing: 10961 - 10980 of 12029 results

  2. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O'Sullivan et al Development. 2024 Aug 1;151(15):dev20284
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Expression
    Mammalian
    Promoter
    GACG and U6.3 chick
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221844-plasmid-map-sequence-id-437475

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  3. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  4. PCDF Bravo-PotH-OL3/2sub

    Plasmid
    #216752
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216752-plasmid-map-sequence-id-434424

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  12. pGBT9-Hook1

    Plasmid
    #198523
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    Hook1 (HOOK1 Human)
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent substitution in codon 620 to eliminate int…
    Promoter
    ADH1
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198523-plasmid-map-sequence-id-389375

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Showing: 10961 - 10980 of 12029 results