We narrowed to 15,772 results for: LEA

1. pCMV-pOTC-3H-GFP

   Plasmid

   #205725

   Purpose

   Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   pOTC-3H-GFP
   

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBlue-TAL

   Plasmid

   #49401

   Purpose

   The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

   Depositor

   Michal Zurovec

   Article

   Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

   Type

   Empty backbone

   Use

   TALEN

   Expression

   Insect

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+ MBIC5-mCer3

   Plasmid

   #74116

   Purpose

   For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

   Depositor

   Michael Tsang

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   MBIC5-mCer3
   

   Use

   Transgenic construct

   Tags

   mCerulean 3

   Promoter

   Myl7 (Cmlc2)

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVITRO1-iRFP702-LaminB1

   Plasmid

   #184456

   Purpose

   Nuclear membrane-targeted iRFP702 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   iRFP702-LaminB1
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBABE-puro-NLS-LSSmKate1

   Plasmid

   #31873

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS-LSS-mKate1
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCY 3010-02

   Plasmid

   #36210

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   yeast-enhanced (yE) polylinker
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-Cerulean-hphMX4

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4252845

   Plasmid

   #153787

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHis-003

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046494

   Plasmid

   #149137

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. CHIKV-nsP3-c001

   Plasmid

   #173086

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   N/A
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A1334-T1493, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS-Syt::3xHalo7

    Plasmid

    #87649

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::3xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::3xHalo7
    

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJY3948

    Plasmid

    #113338

    Purpose

    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

    Depositor

    Suzanne Sandmeyer

    Article

    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

    Insert

    2-pyrone synthase, codon optimized for Y. lipolytica
    

    Tags

    6XHis

    Expression

    Yeast

    Mutation

    Please see depositor comments

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CLAPon

    Plasmid

    #213405

    Purpose

    Peroxidase (APEX) reporter for TEV protease cleavage

    Depositor

    Wenjing Wang

    Article

    Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

    Insert

    E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pISceI-myl7:MBIC5-mCer3

    Plasmid

    #74117

    Purpose

    Zebrafish transgenic construct

    Depositor

    Michael Tsang

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    MBIC5-mCer3
    

    Use

    Mrna generation for microinjection.

    Tags

    mCerulean 3

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046373

    Plasmid

    #145555

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAPV-DsRed2-FLAG-ER-5

    Plasmid

    #170622

    Purpose

    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RECQL5 (RECQL5A-c011)

    Plasmid

    #98231

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RECQL5A (RECQL5 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid

    #182527

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Tags

    4XFLAG

    Expression

    Yeast

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_TALEN-FokI(WT)-BB_V3

    Plasmid

    #42588

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    TALEN-FokI(WT)-BB
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    3XFlag

    Expression

    Mammalian

    Promoter

    CAG hybrid

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge