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B5-5

Plasmid

#111484

Purpose

translational initiation factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 3

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

Plasmid

#99343

Purpose

Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
CBP (340-439)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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PNCA-mCherry

Plasmid

#67790

Purpose

Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

Insert

MMLV-LTR-mCherry

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgMaob-HepmCherry

Plasmid

#192829

Purpose

Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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ALC1-c005

Plasmid

#173092

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

mCerulean

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MacroH2A.2-c002

Plasmid

#173089

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A2 (MACROH2A2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K167-K372

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.2-c002

Plasmid

#173088

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N372, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ALC1-c007

Plasmid

#173112

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

Nanoluc

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c010

Plasmid

#173100

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

F1208-G1388

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PARP9-c024

Plasmid

#173096

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP9 (PARP9 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

G102-K298

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GDAP2-c005

Plasmid

#173110

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GDAP2 (GDAP2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

M1-N231

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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ARH2-c003

Plasmid

#173094

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRHL1 (ADPRHL1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-K354, codon optimized

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBOB-CARMIL2 BH domain-GFP

Plasmid

#118736

Purpose

To express CARMIL2 BH tagged with GFP on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pNC1137

Plasmid

#41563

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-HpDsbG

Plasmid

#39329

Depositor

SeWon Suh

Article

Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

Insert

oxidoreductase

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only