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pMXs-puro-hMGT

Plasmid

#172393

Purpose

Retroviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Publication

Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Retroviral

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Publication

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-C-10

Plasmid

#56224

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Publication

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Publication

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-OLIG2

Plasmid

#134668

Purpose

Retroviral expression of human OLIG2

Depositor

Marius Wernig

Publication

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Publication

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Expression

Mammalian

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PEP102E

Plasmid

#97388

Purpose

binary construct of nuclear-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Publication

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Nuclear target

Expression

Plant

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PEP104E

Plasmid

#97390

Purpose

binary construct of mitochondria-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Publication

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

mitochondria target

Expression

Plant

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PEP106E

Plasmid

#97392

Purpose

binary construct of ER-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Publication

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

ER target

Expression

Plant

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMBD-Gate2

Plasmid

#25947

Depositor

Kamil Onder

Publication

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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fIM1265

Plasmid

#132577

Purpose

pZA2-pLlacO-soxR

Depositor

Timothy Lu

Publication

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLlacO-soxR-TermT1 (soxR )

Expression

Bacterial

Promoter

pLlacO

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJK131

Plasmid

#71548

Purpose

Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

Depositor

T. J. Kappock

Publication

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

Alanine racemase

Expression

Bacterial

Promoter

T7

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Publication

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Publication

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Tags

HaloTag, mGold

Expression

Mammalian

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW

Plasmid

#133382

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Publication

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p10xEGFP-N1

Plasmid

#122170

Purpose

Expression of 10xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

10xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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