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SRSF2 A9.3 gRNA

Plasmid

#90902

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SRSF2 A10.3 gRNA

Plasmid

#90903

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H2.1 gRNA

Plasmid

#90887

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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HJURP E4.1 gRNA

Plasmid

#90711

Purpose

3rd generation lentiviral gRNA plasmid targeting human HJURP

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HJURP (Guide Designation E4.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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BUB3 A10.1 gRNA

Plasmid

#90557

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A10.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330) S278C

Plasmid

#87739

Purpose

Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

Depositor

Chris Hill

Publication

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-277, mutated Ser 278 to Cys

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-HT-Dmp53KRKR

Plasmid

#85699

Purpose

expression of Drosophila p53 KRKR mutant in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Publication

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Tags

His

Expression

Insect

Mutation

K26R K302R

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
xCIAR_pcDNA5/FRT/TO

Plasmid

#86504

Purpose

Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

xCIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

F929A and T968L in SOScat

Promoter

CMV (tet operator)

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Publication

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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BMPR1A gRNA (BRDN0001487137)

Plasmid

#77958

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR1A

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR1A (BMPR1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIM24 gRNA (BRDN0001148301)

Plasmid

#77888

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM24

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K7 gRNA (BRDN0001149362)

Plasmid

#77820

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K7 gRNA (BRDN0001149491)

Plasmid

#77822

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKM gRNA (BRDN0001145396)

Plasmid

#77633

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKM

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKM (PKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001148670)

Plasmid

#77564

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001146365)

Plasmid

#77565

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BMPR1B gRNA (BRDN0001145690)

Plasmid

#77488

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR1B

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR1B (BMPR1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1D gRNA (BRDN0001149348)

Plasmid

#76716

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1D

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1D (CSNK1D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BMPR2 gRNA (BRDN0001144883)

Plasmid

#76391

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR2 (BMPR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CG gRNA (BRDN0001149186)

Plasmid

#76334

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CG

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CG (PIK3CG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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