We narrowed to 19,741 results for: Cre

1. LV_EF1a_CDK6-P2A-Hygro_Barcode

   Plasmid

   #170224

   Purpose

   Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

   Plasmid

   #86017

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Expression

   Mammalian

   Mutation

   Changed threonine 230 to alanine

   Promoter

   CMV IE

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

   Plasmid

   #86018

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Expression

   Mammalian

   Mutation

   Changed aspartic acid 637 to glycine

   Promoter

   CMV IE

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO/SH/GW-Mst2

   Plasmid

   #84300

   Purpose

   Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

   Insert

   Mst2 (STK3 Human)
   

   Tags

   HA, SBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pRD439

   Plasmid

   #168465

   Purpose

   For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mTurquoise2-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1cam-AP-His

   Plasmid

   #71957

   Purpose

   Expresses the extracellular region of the L1CAM protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   L1cam (L1cam Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_HOXA1_P2A_Hygro_Barcode

   Plasmid

   #120451

   Purpose

   Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXA1 (HOXA1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_UdgX-EE-UdgX-NG-nCas9

   Plasmid

   #163570

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AIDx-ID-1058

   Plasmid

   #135347

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-1058-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167848

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti-X1-Neo-DDRGK1-K-less-HA

    Plasmid

    #139849

    Purpose

    Lentiviral expression of human DDRGK1-K-less-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cntn6-AP-His

    Plasmid

    #71944

    Purpose

    Expresses the extracellular region of the Contactin 6 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn6 (Cntn6 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTet-O-ATOH7-T2A-PuroR

    Plasmid

    #162343

    Purpose

    Lentiviral expression of ATOH7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ATOH7 (ATOH7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSico shMAPKAPK3 human

    Plasmid

    #85661

    Purpose

    expression of shRNA targeting human MAPKAPK3

    Depositor

    Julien Sage

    Article

    Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

    Insert

    MAPKAPK3 (MAPKAPK3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Plxnd1-AP-His

    Plasmid

    #72006

    Purpose

    Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnd1 (Plxnd1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. KHBD00517

    Plasmid

    #39618

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5893 (D Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. BMR1_02g02691-bio-His

    Plasmid

    #108042

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_02g02691
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. BMR1_03g01540-bio-His

    Plasmid

    #108043

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_03g01540 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_03g01540
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Puro-sgSIK3-A

    Plasmid

    #138682

    Purpose

    Expresses a mouse SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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