We narrowed to 14,166 results for: flag

1. pEBTetD-SNAP-CPAP

   Plasmid

   #136816

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CENPJ (CENPJ Synthetic, Human)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTetD-SNAP-Cep76

   Plasmid

   #136814

   Purpose

   Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep76 (CEP76 Synthetic, Human)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTetD-SNAP-α-tubulin

   Plasmid

   #136792

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-TUBA (DNMBP Synthetic, Human)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VRER-P2A-Puro

   Plasmid

   #110849

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HF1-P2A-Puro

   Plasmid

   #110850

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-VQR-PGK-Puro

   Plasmid

   #110855

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

   Plasmid

   #101192

   Purpose

   Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K918A/V922A/R925A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

   Plasmid

   #101188

   Purpose

   Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   T657A/G658A/W659A/R661A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid

    #101190

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

    Plasmid

    #101181

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

    Plasmid

    #101182

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid

    #101183

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

    Plasmid

    #101184

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Mutation

    G582A/V583A/E584A/D585A/N588A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid

    #101185

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    G582A/V583A/E584A/D585A/N588A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

    Plasmid

    #101179

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid

    #101180

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

    Plasmid

    #79068

    Purpose

    Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
    

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Changed Lys 283 to Asp and Gln 287 to Asp

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GPR152-Tango

    Plasmid

    #66328

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR152 (GPR152 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only