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We narrowed to 4,504 results for: pcr

Showing: 1141 - 1160 of 4504 results

  1. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  2. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  3. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  4. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  5. pTF265 (pFA6a-PSc-6xGly-12xHis-His3MX)

    Plasmid
    #44063
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44063-plasmid-map-sequence-id-61668

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  6. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Donald Kohn
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198023-plasmid-map-sequence-id-390930

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  7. pTF257 (pFA6a-TEV-6xGly-His3MX)

    Plasmid
    #44068
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44068-plasmid-map-sequence-id-61660

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  8. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid
    #44062
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44062-plasmid-map-sequence-id-61661

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  9. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid
    #44080
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44080-plasmid-map-sequence-id-61664

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  10. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid
    #44074
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44074-plasmid-map-sequence-id-61665

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  11. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid
    #44092
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44092-plasmid-map-sequence-id-61662

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  12. pHyg-PCUP1-AID*(N)

    Plasmid
    #99529
    Purpose
    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Insert
    IAA17(71-117) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Tags
    Expression
    Mutation
    Promoter
    CUP1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99529-plasmid-map-sequence-id-192013

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  13. HOXD9 (human) pET

    Plasmid
    #8570
    Depositor
    Corey Largman
    Insert
    HOXD9 (HOXD9 Human)
    Use
    Tags
    T7
    Expression
    Bacterial
    Mutation
    PCR human cDNA clone; makes protein by in vitro T…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    8570-plasmid-map-sequence-id-208949

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  15. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

    Plasmid
    #198022
    Purpose
    LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population
    Depositor
    Donald Kohn
    Insert
    CD3D c.202C>T-cDNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198022-plasmid-map-sequence-id-390932

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  16. pCCL-c-MNDU3-CD3D-275-2A>G

    Plasmid
    #198024
    Purpose
    LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan
    Depositor
    Donald Kohn
    Insert
    CD3D c.275-2A>G
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198024-plasmid-map-sequence-id-390335

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  19. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

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  20. myc-tagged NRP1 (human)

    Plasmid
    #213068
    Purpose
    Express myc-NRP1 in mammalian cells
    Depositor
    Yoav Henis
    Insert
    neuropilin 1 (NRP1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    N-terminal myc tag was introduced by a three-step…
    Promoter
    T7
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213068-plasmid-map-sequence-id-421637

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Showing: 1141 - 1160 of 4504 results