We narrowed to 14,166 results for: flag

1. pCAG-OSF-PP-human-CHMP3(150)

   Plasmid

   #154181

   Purpose

   expresses human CHMP3(150) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3 (CHMP3 Human)
   

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   C-terminal truncation of CHMP3 at amino acid 150

   Promoter

   CAG

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB-hCRY1-mClover3-CD4-bla

   Plasmid

   #179441

   Purpose

   Donor vector to knock in mClover3 C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mClover3
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL40C_PGKintron_Cas9_Green

   Plasmid

   #134966

   Purpose

   Lentiviral vector coding Cas9 and mNeonGreen

   Depositor

   Beat Bornhauser

   Article

   Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

   Insert

   Cas9-P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   hPGK promoter with beta-globin intron

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1-Mb-Fluoro-Phe B5

   Plasmid

   #197576

   Purpose

   Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

   Inserts

   Mb Pyl Fluoro-Phe B5 synthetase
   Pyl-tRNA (4 copies)
   

   Tags

   Nuclear export sequence + FLAG

   Expression

   Mammalian

   Mutation

   N311G, C313A

   Promoter

   CMV and U6/H1

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A mKate2-hu_ncOGT D554N

   Plasmid

   #154283

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   D554N mutation (Ser/Thr Glycosylation Impaired)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-MAVS(IRSp53 pLxIS)

   Plasmid

   #221315

   Purpose

   Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB-hCRY1-Halo-CD4-bla

   Plasmid

   #179445

   Purpose

   Donor vector to knock in Halotag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   HaloTag (Halo7 Synthetic)
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid

    #154285

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations (HCF-…

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac SpyTag-β2AR-SpyTag

    Plasmid

    #135620

    Purpose

    Expression of SpyTag-B2AR in insect cell

    Depositor

    Hee-Jung Choi

    Article

    Choi et al Science. 2019 Nov 29;366(6469):1150-1156

    Insert

    Beta 2 adrenergic receptor (ADRB2 Human)
    

    Tags

    HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

    Expression

    Insect

    Mutation

    Deleted 1-24 aa, 366-413 aa

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK-ATF4 1-275

    Plasmid

    #26118

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 276-351 have been deleted

    Available Since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

15. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter;

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK-ATF4 111-351

    Plasmid

    #26119

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 1-110 have been deleted

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDEST40-CES2A-ΔC-S227A

    Plasmid

    #200556

    Purpose

    Mammalian expression of secreted mouse CES2A S227A mutant

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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