165,567 results

1. pLX-TRV2

   Plasmid

   #180516

   Purpose

   Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker

   Depositor

   Jose-Antonio Daros

   Article

   Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

   Insert

   Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
   

   Expression

   Plant

   Promoter

   35S CaMV

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T-hLIG3

   Plasmid

   #81055

   Purpose

   bacterial expression of human LIG3

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   DNA ligase 3 (LIG3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-NLS-dCas13-NLS-eNAT10

   Plasmid

   #238300

   Purpose

   Expresses nuclear version of the programmable RNA acetylation system.

   Depositor

   Won Do Heo

   Article

   Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

   Insert

   dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
   

   Use

   CRISPR

   Tags

   SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

   Expression

   Mammalian

   Mutation

   H133A for catalytically inactive mutant, deletion…

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Flag-Tet1 CD

   Plasmid

   #70129

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain.

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. L4440_BioBrick-hsf-1-sgRNA-B

   Plasmid

   #177787

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL99

   Plasmid

   #78577

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Expression

   Bacterial

   Promoter

   inducible PnitA-NitR system

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEZYmyc-His

   Plasmid

   #18701

   Purpose

   pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Tags

   Myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVS1-VIR2

   Plasmid

   #134745

   Purpose

   Virulence and replication helper

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   vir gene cluster
   

   Use

   Vir gene expression

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN-sacB

    Plasmid

    #196249

    Purpose

    Plasmid containing the sacB gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attR2-sacB-attL3
    

    Expression

    Bacterial

    Promoter

    sacB promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV.CBA.eGFP.2A.wtTau

    Plasmid

    #140424

    Purpose

    AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau (MAPT Human, Synthetic)
    

    Use

    AAV

    Tags

    eGFP

    Promoter

    CBA

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-FGFR1c-V5/HIS

    Plasmid

    #201106

    Purpose

    expression of human FGFR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Mutation

    D908A

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2NIL

    Plasmid

    #20188

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology (Reading). 2000 Aug;146 ( P

    Type

    Empty backbone

    Use

    Suicide vector

    Available Since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TLR10-YFP

    Plasmid

    #13643

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 10 (TLR10 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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