162,993 results

1. pAAV-hSyn-con/fon DREADD Gi-mCherry

   Plasmid

   #200660

   Purpose

   Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina

   Depositor

   Samer Hattar

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas13d-BFP

   Plasmid

   #215720

   Purpose

   Catalytically inactive dCasRx with 2A-BFP

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   dCasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH-PanCen-mVenus

   Plasmid

   #49640

   Purpose

   TALEColor-mVenus-Pan-Centromere

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. IKK-2 WT

   Plasmid

   #11103

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-N BHRF1

   Plasmid

   #37937

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BHRF1 (BHRF1 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. KISSPEPTIN-R-DuET

   Plasmid

   #213328

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   KISSPEPTIN-R (KISS1R Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166013

   Purpose

   mammalian expression of MERS-CoV soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   MERS-CoV S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #161927

   Purpose

   Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   Surface marker and Citrine repression reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154787

    Purpose

    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Fly, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and stdMCP

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EF1aL-eGFP-W

    Plasmid

    #126686

    Purpose

    Constitutive, ubiquitous GFP expression.

    Depositor

    Darrell Kotton

    Article

    Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CNR2-DuET

    Plasmid

    #213214

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR2 (CNR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiPGK Puro DEST p38KTRClover

    Plasmid

    #59152

    Purpose

    Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. hrGFP-Keap1

    Plasmid

    #28025

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

    Plasmid

    #208692

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-hRluc

    Plasmid

    #24348

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    hRluc
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL20_mEGFP_TMPRSS2_ETV4

    Plasmid

    #205945

    Purpose

    Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAVU6+27-F30-2xdBroccoli

    Plasmid

    #66842

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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