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(Plasmid #167546)


Item Catalog # Description Quantity Price (USD)
Plasmid 167546 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Luciano Marraffini
  • Backbone size w/o insert (bp) 3729
  • Total vector size (bp) 6411
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
  • Insert Size (bp)
  • Promoter IPTG

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    IPTG promoter and lacI was taken from pPM145, a gift from Luciano Marraffini.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRecT_2 was a gift from Howard Hang (Addgene plasmid # 167546 ; ; RRID:Addgene_167546)
  • For your References section:

    RecT recombinase expression enables efficient gene editing in Enterococcus. Chen V, Griffin ME, Maguin P, Varble A, Hang HC. Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. 10.1128/AEM.00844-21 PubMed 34232061