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(Plasmid #167547)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167547 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Jan-Peter van Pijkeren
  • Backbone size w/o insert (bp) 9747
  • Total vector size (bp) 10249
  • Modifications to backbone
    Erythromycin resistance was swapped for Chloramphenicol via chloramphenicol acetyl transferase. pUC19 origin or replication was inserted.
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 12.5 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
    chloramphenicol acetyl transferase
  • Alt name
  • Insert Size (bp)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    pUC19 origin of replication
  • Alt name
  • Insert Size (bp)

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pUC19 origin of replication was cloned from pUC19 obtained from New England Biolabs. Chloramphenicol acetyltransferase was cloned from pKH12, a gift from Kelli Palmer.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9 was a gift from Howard Hang (Addgene plasmid # 167547 ; ; RRID:Addgene_167547)
  • For your References section:

    RecT recombinase expression enables efficient gene editing in Enterococcus. Chen V, Griffin ME, Maguin P, Varble A, Hang HC. Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. 10.1128/AEM.00844-21 PubMed 34232061