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RecT recombinase expression enables efficient gene editing in Enterococcus.
Chen V, Griffin ME, Maguin P, Varble A, Hang HC
Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
167546pRecT_2Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
167547pCas9Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Antibodies from Article