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Rab2a cds in pGem-T Easy

Plasmid

#86219

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab2a (rab2a Zebrafish)

Use

In situ probe/other

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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Rab3ab cds in pGem-T Easy

Plasmid

#80509

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab3ab (rab3ab Zebrafish)

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab6ba cds in pGem-T Easy

Plasmid

#80520

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab6ba transcript variant X1 (rab6ba Zebrafish)

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.M35I

Plasmid

#82818

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PTEN (PTEN Human)

Use

Gateway Cloning

Mutation

M35I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Rab25a cds in pGem-T Easy

Plasmid

#80544

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab25a (rab25a Zebrafish)

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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Rab32a cds in pGem-T Easy

Plasmid

#80549

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab32a (rab32a Zebrafish)

Available since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW009

Plasmid

#83523

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene

Depositor

Claudia Vickers

Publication

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha1

Expression

Yeast

Promoter

pARO9

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW005

Plasmid

#83524

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

Depositor

Claudia Vickers

Publication

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-malpha1

Expression

Yeast

Promoter

pFUS1

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJKW 0633

Plasmid

#74122

Purpose

Photinus pyralis sulfotransferase ST2 - pHis8-4

Depositor

Jing-Ke Weng

Publication

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis sulfotransferase 2

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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hFLN29G37-end/pCMV-14-FLAG

Plasmid

#73708

Purpose

Express human TRAFD1 (FLN29) from G37 to end in mammalian cells

Depositor

Paul Odgren

Publication

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 36 aa from full lenght TRAFD1

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pBAD-TRP32

Plasmid

#71730

Purpose

inducible expression of TRP32 in E.coli

Depositor

Jere McBride

Publication

Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

Insert

TRP32

Tags

6xHIS and Thioredoxin

Expression

Bacterial

Promoter

pBAD

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
Gal4pBS2-Pleum

Plasmid

#59992

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mPapaya-Vimentin-7

Plasmid

#56646

Purpose

Localization: Intermediate Filaments, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Vimentin (VIM Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-LC-Myosin-C-10

Plasmid

#56402

Purpose

Localization: Light Chain Myosin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

LC-Myosin

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTYB-pLAC-F112S

Plasmid

#48727

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

Depositor

Gordon Laurie

Publication

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Phenylalanine 12 to Serine

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I98S

Plasmid

#48723

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

Depositor

Gordon Laurie

Publication

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 98 to Serine

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I73S

Plasmid

#48722

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Publication

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 73 to Serine

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-V69S

Plasmid

#48721

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

Depositor

Gordon Laurie

Publication

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Valine 69 to Serine

Promoter

T7

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only
TAL3521

Plasmid

#47240

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-prrx1a-right (prrx1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3520

Plasmid

#47239

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-prrx1a-left (prrx1a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only