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pEBG-Pak1 83-149

Plasmid

#12214

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Mammalian

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Del 3)

Plasmid

#16603

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 3) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-3 (AccI->PvuII). The AccI site is at -741 …

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
Mito-mhYFP

Plasmid

#186526

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin S518A

Plasmid

#84294

Purpose

Expression of Merlin with an alanine mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518A

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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wt dynamin 1 pEGFP

Plasmid

#34680

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBV163

Plasmid

#108364

Purpose

Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

Depositor

Scott Moye-Rowley

Article

Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

Insert

Knock-in Cg TDH3 promoter in Cg YAP1 gene

Use

Replace c.glabrata yap1 promoter with c. glabrata…

Promoter

TDH3

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-N2c_envA-IRES-Neo

Plasmid

#172368

Purpose

Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

envA + Neo

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin S518D

Plasmid

#84295

Purpose

Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518D

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-1

Plasmid

#72343

Purpose

Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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UKNP3.1.2

Plasmid

#98215

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-H3.3-N-14

Plasmid

#55302

Purpose

Localization: Nucleus/Histones, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

H3.3 (H3f3b Mouse)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBSFI-myr-akt1

Plasmid

#49186

Purpose

cDNA of Akt1 with myristyolation signal in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1deltaP

Plasmid

#35030

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DELTAP (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
HA-E2F-1 wt-pRcCMV

Plasmid

#21667

Depositor

William Kaelin

Article

Krek et al Cell. 1994 Jul 15. 78(1):161-72.

Insert

E2F-1 (E2F1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type-

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pRRL CAG NFlag BAF170 ires GFP

Plasmid

#24562

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF170 (Smarcc2 Mouse)

Use

Lentiviral

Tags

Flag

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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