Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status.
Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. Epub 2023 Jun 7. PubMed Article
Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. Epub 2023 Jun 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203479 | pGWB511-TP-UnaG-FLAG | For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter. |
| 203480 | pGWB511-TP-BVRA | For Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter. |
| 203482 | pGWB511-TP-tagRFP | For Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter. |
| 203483 | pGWB602-TP-mCherry-UnaG | For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter. |
| 203484 | pGWT35S-TP-UnaG | For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter. |
| 203485 | pDEST17-UnaG | For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter. |
| 203486 | pDEST17-HO1(51-231) | For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter. |
| 203760 | pGWB560-NADK2 | For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter. |