We narrowed to 14,504 results for: flag

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pSDC803_IKZF1_mutant

Plasmid

#224113

Purpose

Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

IKZF1 (IKZF1 Human)

Tags

Flag-TEV

Expression

Insect

Mutation

Residues 140-196, Q146A and G151N

Promoter

polyhedrin

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS and P2A

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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CXCR4-Tango

Plasmid

#66262

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD

Plasmid

#37188

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

CMV

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti PGK mKate2-OGT WT (Puro)

Plasmid

#154290

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

Dec. 31, 2020

Availability

Academic Institutions and Nonprofits only

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LPAR1-Tango

Plasmid

#66418

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

LPAR1 (LPAR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1c-dCas9

Plasmid

#232178

Purpose

Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 Y254C

Plasmid

#231587

Purpose

ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed tyrosine 254 to cysteine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

Plasmid

#121538

Purpose

An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV2 and AAV5

Insert

HA-tagged hM4D(Gi) (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

SYN1

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGHUC w-ERBIN (2-hybrid prey plasmid)

Plasmid

#128163

Purpose

UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

Depositor

Marcus Noyes

Article

Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

Insert

ERBIN PDZ domain (ERBIN Synthetic)

Use

Synthetic Biology

Tags

FLAG and Omega subunit of RNA polymerase

Expression

Bacterial

Promoter

UV5

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1

Plasmid

#35170

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-Tbg-CES2A-ΔC

Plasmid

#200558

Purpose

Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-dCas9-D3A

Plasmid

#78254

Purpose

Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Article

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

Plasmid

#209194

Purpose

Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

Depositor

Larry Zweifel

Article

Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

Insert

Kcnq3 (KCNQ3 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

R230 to C230

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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