We narrowed to 19,744 results for: Cre

1. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

   Plasmid

   #105024

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfb (Cobra1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121842

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

   Plasmid

   #114088

   Purpose

   U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for MbCas12a crRNAs
   

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-shCasp2-IRES-GFP

   Plasmid

   #52061

   Purpose

   stable knockdown of Caspase-2 in mammalian cells

   Depositor

   Tyler Jacks, Trudy Oliver

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Caspase-2 shRNA (CASP2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pFUSEss-CHIg-mG3_CH3-1

   Plasmid

   #105859

   Purpose

   Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG1, IgG3 (Ighg1, Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   hybrid heavy chain of mouse IgG3 with CH3 derived…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSEss-CHIg-mG3_CH2-1

   Plasmid

   #105857

   Purpose

   Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG1, IgG3 (Ighg1, Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   hybrid heavy chain of mouse IgG3 with CH2 derived…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUSEss-CHIg-mG3_CH1h-1

   Plasmid

   #105856

   Purpose

   Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG1, IgG3 (Ighg1, Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   hybrid heavy chain of mouse IgG3 with CH1 and hin…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-218_HNF4A2

   Plasmid

   #31090

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   splice variant 2 of HNF4A, deletion of CMV promo…

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-138_HNF4A8

   Plasmid

   #31093

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   splice variant 8 of HNF4A, deletion of CMV promo…

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CHAF1B_pLENTI-CAG-IRES-GFP

    Plasmid

    #177009

    Purpose

    Mammalian lentiviral expression vector encoding CHAF1B

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CHAF1B (CHAF1B Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-68_HNF4A2

    Plasmid

    #31092

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 2 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUSEss-CHIg-mG3_h-1

    Plasmid

    #105861

    Purpose

    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived hinge.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    

    Expression

    Mammalian

    Mutation

    hybrid heavy chain of mouse IgG3 with hinge deriv…

    Promoter

    hEF1-HTLV prom

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86023

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain: Changed threonine 230 to…

    Promoter

    CMV IE

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Expression

    Bacterial

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86024

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain: Changed aspartic acid 63…

    Promoter

    CMV IE

    Available Since

    April 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS L30 Ruby3-Rab7A

    Plasmid

    #135651

    Purpose

    Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    Ruby3

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTT5-hSDC1-CS2

    Plasmid

    #52367

    Purpose

    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R95Q and S194A (according to numbering in Fig. 2A…

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only