We narrowed to 19,744 results for: Cre

1. pENTR1A 2xHA LTB4R

   Plasmid

   #226806

   Purpose

   Gateway entry vector for 2xHA LTB4R

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Mutation

   Sequence optimized from NP_858043.1

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer20 2xHA GPR137

   Plasmid

   #226813

   Purpose

   Tet-inducible lentiviral vector for 2xHA GPR137 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR137 (GPR137 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Mutation

   Sequence optimized from NP_001164351

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOGS539_GLuc

   Plasmid

   #226718

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

   Plasmid

   #226989

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

   Plasmid

   #226991

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

   Plasmid

   #226990

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CMV_Puro_SFB_GNB1L

   Plasmid

   #224381

   Purpose

   Lenti plasmid for generating GNB1L expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_TELO2_gRNA

   Plasmid

   #224379

   Purpose

   Targets TELO2 for dTAG N terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   TELO2 (TELO2 Human)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459_GNB1L_gRNA

    Plasmid

    #224377

    Purpose

    Targets GNB1L for dTAG C terminal knock-in cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-CRT-Fc P

    Plasmid

    #221855

    Purpose

    Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses P domain

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-CRT-Fc N

    Plasmid

    #221854

    Purpose

    Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses N domain

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-CRT-Fc C

    Plasmid

    #221856

    Purpose

    Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses C domain

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-miniTurbo-TAF5-FL

    Plasmid

    #209051

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-3xFLAG-EGFP

    Plasmid

    #209058

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged EGFP

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    EGFP
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-miniTurbo-EGFP

    Plasmid

    #209059

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to EGFP

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    EGFP
    

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-3xFLAG-TAF5-FL

    Plasmid

    #209049

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKOv3-intergenic-As

    Plasmid

    #209026

    Purpose

    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCHKOv3-CD46 Ex3_1-Lb

    Plasmid

    #209027

    Purpose

    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKOv3-CD46 Ex3_2-Lb

    Plasmid

    #209028

    Purpose

    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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