Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 36,889 results for: kan

Showing: 11941 - 11960 of 36889 results

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Publication

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Publication

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC-K

Plasmid

#174581

Purpose

pOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistant

Depositor

Mark Banfield

Type

Empty backbone

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Publication

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Publication

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2 PUb-GFP

Plasmid

#173667

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Publication

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
EGFP

Expression

Insect

Mutation

Nix isoforms with only exons 1, 2 separated by in…

Promoter

Aedes aegypti Polyubiquitin and cloned Nix promot…

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SFXN1_STOP

Plasmid

#161477

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SFXN1 (SFXN1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SV2C_STOP

Plasmid

#161463

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2C (SV2C Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-UNC93B1_STOP

Plasmid

#161464

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UNC93B1 (UNC93B1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC25A1_STOP

Plasmid

#161465

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A1 (SLC25A1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC7A3_STOP

Plasmid

#161467

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SV2B_STOP

Plasmid

#161447

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2B (SV2B Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC4A4_STOP

Plasmid

#161426

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A4 (SLC4A4 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC7A11_STOP

Plasmid

#161410

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC7A8_STOP

Plasmid

#161399

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A8 (SLC7A8 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC47A1_STOP

Plasmid

#161393

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC47A1 (SLC47A1 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC5A8_STOP

Plasmid

#161384

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A8 (SLC5A8 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC6A9_STOP

Plasmid

#161386

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A9 (SLC6A9 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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