Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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HRas (G12V, E37G)-pcw107-V5

Plasmid

#64640

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Tags

V5

Mutation

G12V, E37G

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSC-hPAX8_tdTomato-PGK-PuroTK

Plasmid

#206004

Purpose

plasmid to insert tdTomato reporter at the 3' end of the human PAX8 gene

Depositor

Darrell Kotton

Publication

Undeutsch et al Stem Cell Reports. 2024 Dec 10;19(12):16

Inserts

PAX8 5' homology arm, P2A-tdTomato (PAX8 Human)
lox-P - PGK - puroTK - loxP
PAX8 3' homology arm (PAX8 Human)

Expression

Mammalian

Promoter

PGK

Available since

Oct. 8, 2026

Availability

Academic Institutions and Nonprofits only

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