We narrowed to 4,734 results for: Drosophila

1. K560GFP

   Plasmid

   #129761

   Purpose

   Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET1.2-STOP-dsRed

   Plasmid

   #60944

   Purpose

   Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   dsRED
   

   Use

   Unspecified

   Promoter

   3XP3

   Available Since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEntr_PE2

   Plasmid

   #149548

   Purpose

   Gateway entry clone with PE2 enzyme (with stop codon)

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR; Gateway shuttle vector

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Promoter

   vasa

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAct-GW-HygroR

   Plasmid

   #149610

   Purpose

   Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   Gateway cassette (ccdb + Chlor.R.)
   

   Expression

   Insect

   Promoter

   Act5c

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. KRigor980

   Plasmid

   #129763

   Purpose

   Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R210A

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-HA-STX1

   Plasmid

   #89310

   Purpose

   Expresses HA tagged syntaxin 1 in Drosophila cells

   Depositor

   Andrew Peden

   Insert

   STX1 (Syx1A Fly)
   

   Tags

   HA tag

   Expression

   Insect

   Promoter

   P-MT (Metallothionein)

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. K560_AviC_coBirA

   Plasmid

   #129765

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT-TAK1-V5-His

   Plasmid

   #63753

   Purpose

   Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   TGF-β activated kinase 1 (Tak1 Fly)
   

   Tags

   V5-His

   Expression

   Insect

   Promoter

   pMT

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Tags

    NLS-3xHA

    Expression

    Insect

    Promoter

    act5C

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACU

    Plasmid

    #58373

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSTEP

    Plasmid

    #72334

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hy_pMT EGFP SV40 pA

    Plasmid

    #69911

    Purpose

    Expresses EGFP in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDsRed-TSTEP_v2

    Plasmid

    #72336

    Purpose

    Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)

    Depositor

    Avital Rodal

    Article

    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

    Insert

    RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
    

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CvcE in pGEX4t

    Plasmid

    #112977

    Purpose

    For protein expression and purification of Drosophila CvcE

    Depositor

    Terry Orr-Weaver

    Insert

    cycE (CycE Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. CJ52 (cg1849 utr mut)

    Plasmid

    #17780

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg1849 utr fragment mut
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    mutant version

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. K406

    Plasmid

    #129764

    Purpose

    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 406

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CJ53 (cg5166a/c utr)

    Plasmid

    #17781

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg5166a/c utr fragment
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pAc5.1B-EGFP-DmEDC3

    Plasmid

    #21713

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

    Insert

    Enhancer of decapping 3 (Edc3 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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