We narrowed to 623 results for: lbr

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pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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FB026

Plasmid

#119711

Purpose

Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter gpdA:YFP coding sequence:Terminator trpC

Use

Synthetic Biology

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.tetO.Nurr1

Plasmid

#234848

Purpose

3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Nurr1 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Rat, Mouse, Bovine, Human)

Tags

Nt: histag-3C protease site

Expression

Bacterial

Promoter

T7 polymerase

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Rat, Mouse, Bovine, Human)

Tags

his and signal sequence for secretion

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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FB013

Plasmid

#119710

Purpose

TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

Plasmid

#234846

Purpose

3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMG10

Plasmid

#31238

Depositor

Keith Joung

Article

Soellu et al Nucleic Acids Res. 2010 Dec 1. 38(22):82

Type

Empty backbone

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJC53.2/PRMT5

Plasmid

#35692

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 5

Promoter

Sp6 for sense, T7 for sense and antisense

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMH4-SYN-ChR2-tdimer2RFP

Plasmid

#22772

Depositor

Thomas Oertner

Article

Zhang et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

ChR2-tdimer2RFP

Tags

ChR2-tdimer2RFP

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF4b

Plasmid

#17998

Depositor

Robert Tjian

Article

Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

Insert

hTAF4b (TAF4B Human)

Tags

HA

Expression

Mammalian

Mutation

aa1-64 deleted

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only