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Plasmid#187425PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorUseTagsExpressionInsectMutationchanged arginine at 118 to glutamic acidPromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hSyn-ASAP3Kv-WPRE
Plasmid#132332Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3Kv-WPRE
Plasmid#132330Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3KV
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-FOXH1
Plasmid#101431PurposeDonor Vector containing FOXH1 transcription factor, part of the Human TFome CollectionDepositorInsertFOXH1 (FOXH1 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGC-I2Cas9
Plasmid#173158PurposeExpresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.DepositorInsertshSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region
UseTags3xFLAG-NLS and NLSExpressionPlantMutationContains a D169N mutation with no visible effect …PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGL4.83(hRlucP/Puro)
Plasmid#212934PurposeRenilla luciferase+PEST vector for investigating faster response to transcriptional changesDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-795
Plasmid#161881PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsMBPExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-633
Plasmid#161877PurposeStandard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsHis6-GSTExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHP1049 - pFB-YCK2
Plasmid#32433DepositorInsertYCK2 (YCK2 Budding Yeast)
UseTagsExpressionInsectMutationY193LPromoterP-P10 and P-PolAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
QPRTA-c002
Plasmid#205115PurposeQPRT protein expressionDepositorInsertQPRT (QPRT Human)
UseTagsTEV-6HisExpressionInsectMutationWTPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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LHP1048 - pFB-RSP5
Plasmid#32434DepositorInsertRSP5 (RSP5 Budding Yeast)
UseTagsExpressionInsectMutationPromoterP-P10 and P-PolAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER3R
Plasmid#185883PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
RENBP_11-427_BioN
Plasmid#195869PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRENBP (RENBP Human)
UsePfbd-bira, baculovirus expressionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only