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AiP1100 - pAAV-AiE0339m-minBG-FlpO-WPRE-HGHpA

Plasmid

#230571

Purpose

AiE0339m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO

Use

AAV

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29R-rat IgG2a] - Chimeric

Plasmid

#231821

Purpose

Mammalian expression plasmid of anti-Neuroligin-3 (Rat) rat IgG2a R-mab. Derived from hybridoma N110/29.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Publication

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 H1101D (GAC)

Plasmid

#212427

Purpose

Encodes SARS-CoV-2 spike H1101D for pseudovirus production

Depositor

Alejandro Balazs

Publication

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike H1101D (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

H1101D

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_W

Plasmid

#147897

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

10 silent mutations and two non silent N605S, K75…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_W

Plasmid

#147898

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-NF1144AA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation and one non silent mutation R23…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W

Plasmid

#147899

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation and one non silent mutation R23…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186708

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Publication

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

PpsbA2*

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186710

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Publication

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

Ptrc.x.tetO2

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

Plasmid

#238327

Purpose

ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E576A-K623A-Q625S-K627A

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG

Plasmid

#240139

Purpose

Express CDK11 p110B D543N mutant

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Publication

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

CDK11 p110 (CDK11A Human)

Tags

FLAG

Expression

Mammalian

Mutation

D543N

Promoter

TRE, CMV

Available since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

Plasmid

#238325

Purpose

ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K558A-K605A-K732A-K817A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233402

Purpose

_-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W

Plasmid

#147886

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

Plasmid

#147887

Purpose

Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]

Plasmid

#107841

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of C-terminal domain in splicing reporter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]

Plasmid

#107840

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of RRM domains in splicing reporter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Plasmid

#238314

Purpose

ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

Plasmid

#39303

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q109A, W110A, K112A and K113A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

Plasmid

#242599

Purpose

A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)

Expression

Mammalian

Promoter

human EF1a

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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