159,332 results

1. pcDNA-R-iLACCO1

   Plasmid

   #208025

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pγsat

   Plasmid

   #39238

   Depositor

   Niall Dillon

   Article

   Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

   Insert

   8 copies of the 234bp mouse γ-satellite repeat
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

   Plasmid

   #182926

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5B-TGFbeta receptor II wt

   Plasmid

   #11766

   Depositor

   Jeff Wrana, Joan Massague

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLH-sgRNA1-2XPP7

   Plasmid

   #75390

   Purpose

   sgRNA1-2XPP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-GR

   Plasmid

   #215094

   Purpose

   Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   NR3C1 (NR3C1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BSD-CryC

   Plasmid

   #209672

   Purpose

   Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

   Depositor

   Sanjeevi Sivasankar

   Article

   Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Lentiviral

   Tags

   Split-TurboID-C and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT3L-12

   Plasmid

   #13736

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   SIRT3 (SIRT3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

   Plasmid

   #229794

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExp-MsyB

    Plasmid

    #112570

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-Neo/Myc PD-L1

    Plasmid

    #121477

    Purpose

    expression of human Myc-PD-L1 in mamalian cell

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Solo-mStayGold-TJP1

    Plasmid

    #227300

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-TJP1

    Plasmid

    #227299

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1.509

    Plasmid

    #203955

    Purpose

    Level 1 self-replicating vector containing a DAPG inducible FnCas12a

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    PphlF-Cpf1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EFIa-Id1-PGK-puro

    Plasmid

    #107735

    Purpose

    Expresses Id1 in mammalian cells

    Depositor

    Alexandre Colas

    Article

    Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

    Insert

    Id1 (T Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLXRN-EGFP

    Plasmid

    #99207

    Purpose

    EGFP expressing bicistronic retroviral vector

    Depositor

    Peter Pedersen

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A Kozak sequence was incorporated to enhance tran…

    Promoter

    LTR

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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