Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,583 results for: golden gate

Showing: 1601 - 1620 of 3583 results

  8. DVA_p15A_AH

    Plasmid
    #217602
    Purpose
    Destination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217602-plasmid-map-sequence-id-468587

    Enlarge

  9. pSF511

    Plasmid
    #222719
    Purpose
    CRISPR/Cas9 plasmid with gRNA1 for site gsdA, Cas9
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    gRNA1(gsdA)
    Use
    CRISPR; A. niger
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pmbfA
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    222719-plasmid-map-sequence-id-467742

    Enlarge

  10. pSF522

    Plasmid
    #222588
    Purpose
    BB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locus
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    A. niger
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222588-plasmid-map-sequence-id-450454

    Enlarge

  11. pSF2

    Plasmid
    #222590
    Purpose
    gsdA disruption cassette via integration of pyrG from A. nidulans
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    pyrG
    Use
    A. niger
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222590-plasmid-map-sequence-id-443810

    Enlarge

  12. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

    Enlarge

  13. pAIP4_6F2Q

    Plasmid
    #234224
    Purpose
    Heterologous protein expression of galectin-3 (Gal-3) (CBP 35) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6F2Q (LGALS3 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234224-plasmid-map-sequence-id-464189

    Enlarge

  14. pAIP4_2JHX

    Plasmid
    #234218
    Purpose
    Heterologous protein expression of rho GDP-dissociation inhibitor 1 (Rho GDI alpha) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2JHX (ARHGDIA Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234218-plasmid-map-sequence-id-464183

    Enlarge

  15. pAIP4_5HFD

    Plasmid
    #234189
    Purpose
    Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HFD (Dlg4 Rat)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234189-plasmid-map-sequence-id-464154

    Enlarge

  16. pAIP4_2PPN

    Plasmid
    #234190
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2PPN (FKBP1A Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234190-plasmid-map-sequence-id-464155

    Enlarge

  17. pAIP4_5MC7

    Plasmid
    #234155
    Purpose
    Heterologous protein expression of coatomer subunit zeta-1 (Zeta-1-coat protein) (Zeta-1 COP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5MC7 (COPZ1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234155-plasmid-map-sequence-id-464121

    Enlarge

  19. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Use
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228663-plasmid-map-sequence-id-461604

    Enlarge

Showing: 1601 - 1620 of 3583 results