We narrowed to 4,504 results for: pcr

1. pHyg-AID(1-114)

   Plasmid

   #99513

   Purpose

   Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(1-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   Expression

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Promoter

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTOPO-col10a1-KI-donor

   Plasmid

   #184874

   Purpose

   Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

   Depositor

   Christoph Winkler

   Article

   Hui et al Development. 2022 May 20. pii: 275483. d

   Inserts

   Collagen10a1 5' homology arm
   Collagen10a1 3' homology arm
   p2a-CreERT2
   Myosin light chain 2 promoter
   eGFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-D-kcnj13

   Plasmid

   #173143

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-D-kcnj1b

   Plasmid

   #173126

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj1b (kcnj1b Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-kcnj8

   Plasmid

   #173134

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTOPO-HqERV(del5'-LTR)

   Plasmid

   #164089

   Purpose

   transfection of Hq-ERV without 5'-LTR

   Depositor

   Maik Hintze

   Article

   Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

   Insert

   Hq-ERV
   

   Use

   Eukaryotic expression vector

   Tags

   Expression

   Mutation

   deletion of 5'-LTR

   Promoter

   none (5'-LTR deleted)

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. HoxC-downstream-5xTetO Donor

   Plasmid

   #131341

   Purpose

   Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   HoxC-downstream-5xTetO Donor
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Y365A-GFP Donor for iMTr EED

   Plasmid

   #131325

   Purpose

   Donor to generate i-MT-r system

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   i-MT-r.Donor
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. A365Y-GFP Donor for iWTr EED

    Plasmid

    #131324

    Purpose

    Donor to generate i-WT-r system

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    i-WT-r.Donor
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJET1.2_Rab28_Sense

    Plasmid

    #124441

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHyg-AID(31-114)

    Plasmid

    #99514

    Purpose

    Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(31-114) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    Expression

    Mutation

    internal inversion of nucleotides 265-312 of the …

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-ARF4-no stop

    Plasmid

    #67311

    Purpose

    hArf4 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR26

    Plasmid

    #127372

    Purpose

    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104912

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104908

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104906

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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