We narrowed to 3,158 results for: multiplex

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hCMV-IE1:NLuc

Plasmid

#118065

Purpose

Transcriptional unit for constitutive expression of NLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:NLuc:bGH

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GeNL_SV40:PuroR

Plasmid

#183040

Purpose

Expresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ143-ZmUbi-tRNA

Plasmid

#158400

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:GrRenilla

Plasmid

#118067

Purpose

Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:ELuc

Plasmid

#118062

Purpose

Transcriptional unit for constitutive expression of ELuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:ELuc:bGH

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-tRNA

Plasmid

#158402

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
STAGR_gRNAScaffold_hH1

Plasmid

#102841

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_hH1

Use

Pcr template for stagr inserts

Promoter

hH1

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_Neo

Plasmid

#102992

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p5xNF-kb_RE

Plasmid

#118050

Purpose

GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

5 copies of the NF-kb DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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ePPEmax*

Plasmid

#205244

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SPDK3860

Plasmid

#199245

Purpose

Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

pPEBV

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-CeNL_SV40:PuroR

Plasmid

#183039

Purpose

Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

CeNL Nanolantern

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-YeNL_SV40:PuroR

Plasmid

#183041

Purpose

Expresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

YeNL Nanolantern

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OeNL_SV40:PuroR

Plasmid

#183042

Purpose

Expresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge