We narrowed to 14,884 results for: pET

1. pET-28a(+)-FGF1-4X

   Plasmid

   #120282

   Purpose

   Produce human recombinant FGF1-4X in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 1 mutant
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21 gamma-tubulin

   Plasmid

   #101825

   Purpose

   production of gamma-tubulin in bacteria

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Whole protein

   Promoter

   T7

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-msfGFP-NPM1

   Plasmid

   #237670

   Purpose

   For expression of msfGFP-NPM1 in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Huimin Zhao, Jin Wang

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET3a-OSF-CypA

   Plasmid

   #79039

   Purpose

   Bacterial vector for cloning and propagation in DH5alpha

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BirA in pET28a (w400-2)

   Plasmid

   #26624

   Depositor

   Eric Campeau

   Insert

   BirA
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid

    #117656

    Purpose

    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Inserts

    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaMIgG-14

    Plasmid

    #228994

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-14
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-SUMO-hGSDMD

    Plasmid

    #111559

    Purpose

    Express human GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (GSDMD Human)
    

    Use

    Tags

    His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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